Effect of Apoptin Mediated by Attenuated Salmonella typhimurium Type Ⅲ Secretion System on Glycolysis in Tumor Cells
YU Chuan
SHI Shengli
ZHU Wenwen
HU Lizhong
SONG Minjie
LI Jingya
ZHANG Chunjie
Abstract：To further investigate the effect of Apoptin mediated by recombinant attenuated Salmonella typhimurium type Ⅲ secretion system on glycolysis of tumor cells,B16F10 cells were infected with recombinant strain∆crp∆asd SL1344(pYA3493-SopE-Apoptin)or complementary strain∆crp∆asd SL1344(pYA3493-SopE),and the cells were collected at 12 h and 24 h after infection to detect the levels of pyruvic acid,lactic acid,pyruvate kinase and hexokinase.The results showed that the intracellular pyruvate level of recombinant strain group was significantly reduced(P<0.01).At 24 h after infection,the lactic acid content of recombinant strain group was significantly lower than that of complementary strain group and cell control group(P<0.01).In addition,the pyruvate kinase activity and hexokinase activity of recombinant strain group were significantly lower than that of complementary strain group and cell control group(P<0.05).These results suggest that recombinant strain can inhibit the glycolysis ability of B16F10 cells by down-regulating the pyruvate and lactic acid content and reducing the activities of pyruvate kinase and hexokinase,leading to significant metabolic changes in tumor cells.
Keywords：ApoptinGlycolysisAttenuated Salmonella typhimuriumApoptosisTumor cells
Publication Date：2023-12-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 136-141 )