Effect of Combinations with Different Concentrations of IAA and 6-BA on Shoot Tip Culture and Phenotypic Variation of Sweetpotato
JIA Licong
WANG Lianjun
YANG Zitong
SHANG Lili
ZHANG Lei
QIU Pengfei
WANG Cuijuan
HAN Junjie
XIN Guosheng
Abstract：The shoot tips of sweetpotato variety Yanshu 29 were used as explants and MS medium as basic medium to study the callus induction,buds differentiation,plants regeneration and phenotypic variation under different concentrations of indole-3-acetic acid(IAA)(0.1 and 0.5 mg/L)and 6-benzylaminopurine(6-BA)(0.1,0.5 and 1.0 mg/L).The results showed that when the IAA concentration in the basic MS medium was 0.5 mg/L,the callus induction effect was the best,shoot tip survival rate was the highest,and the average callus induction rate and shoot tip survival rate of Yanshu 29 were 92.00% and 88.00%,respectively.The bud induction rate and tufted buds induction rate generally increased with the increase of 6-BA concentration in the medium.Under 0.1 mg/L IAA,the average induction rate of buds and tufted buds reached 64.27% and 80.56%,respectively.Addition of 0.5 mg/L IAA in medium was beneficial to plants regeneration,with the average plant regeneration rate of 39.33%;under this IAA concentration,the plant regeneration rate and number of regeneration plant increased,and plant regeneration cycle decreased with the increase of 6-BA concentration.A somaclonal variation line,named YM6-11,was found from regeneration plants,and its leaf vein color,flower shape and cross-compatibility were different compared with Yanshu 29.In conclusion,the most suitable medium for shoot tip culture of Yanshu 29 is MS+0.5 mg/L IAA+1.0 mg/L 6-BA,and the phenotypic and genetic identification should be performed when the regenerated plants are used for actual production,so as to reduce unnecessary economic losses.
Keywords：SweetpotatoShoot tip cultureCallus inductionPlant regenerationSomaclonal variationIAA6-BA
Publication Date：2023-12-15
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：7( 42-48 )