Polymorphism Analysis of Sheep Pre-miR-1
ZHANG Xiangyu
WU Yuyu
NIU Sunmin
CUI Yulong
HU Shengwei
NI Wei
Abstract：To study the single nucleotide polymorphism in pre-miR-1 among sheep breeds with different meat production characteristics and analyze its association with skeletal muscle development, an asymmetric PCR-SSCP technique was used to detect SNP of pre-miR-1 sequences in sheep breeds with different meat production characteristics, including Suffolk sheep and Hu sheep, and the effect of SNP sites on the secondary structure and stability of pre-miR-1 was predicted.The experimental results showed that an A→G mutation was detected at 115 bp downstream of the mature sequence of miR-1, and the SNP locus had a certain correlation with the meat production performance of sheep.In Suffolk sheep, an excellent mutton sheep breed imported from abroad, GG is the dominant genotype.The G allele frequency reaches 0.76.The prediction of the secondary structure of pre-miR-1 indicated that the polymorphism site caused a change in the secondary structure of pre-miR-1 and theΔG decreased by 1.5 kJ/mol.In conclusion, the SNP in pre-miR-1 may have a significant impact on the processing of miR-1, and affects the performance of sheep meat production.
Keywords：SheepPre-miR-1Skeletal muscleAsymmetric PCR-SSCPPolymorphism
Publication Date：2018-12-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 121-125 )