Identification and Biological Characteristics of the Pathogen of Lily Bulb Rot Disease
CAO Xing
LIU Nannan
HU Yanpei
YI Mingfang
WANG Guiqing
Abstract：To determine the pathogen causing lily bulb rot disease in Changping district, Beijing city, the pathogens were identified by tissue isolation, pathogenicity test, morphological characteristics study and r DNA-ITS sequence analysis.Biological characteristics of the major pathogen were determined by measuring the diameter of the colony and counting the number of conidia using hemocytometer measurement.The results showed that Fusarium oxysporum and Fusarium solani were identified as the pathogens causing bulb rot in lily.F.oxysporum was the dominant strain although its pathogenicity was weaker than F.solani.For the mycelial growth and sporulation of F.oxysporum, the optimum medium was PDA, the optimum temperature was 25℃, and the optimum light condition was 12∶12 alternation of light and darkness.The optimal pH values for mycelial growth and sporulation were 5 and 7, respectively.Lactose was the best carbon source and NaNO3was the best nitrogen source for mycelial growth.The optimum carbon source and nitrogen source for sporulation were glucose and glycine separately.
Keywords：LilyBulb rot diseasePathogen identificationFusarium oxysporumBiological characteristics
Publication Date：2018-12-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 96-101 )