Correlation of Monoterpenes Release and LhTPS Expression in Lilium Plants
WANG Ting
FAN Junmiao
ZHANG Haoyu
FAN Weixin
DU Fang
Abstract:The aim of this experiment was to analyze the correlation of the monoterpenes release and monoterpene synthase gene expression in Lilium plants.Floral scents emitted from 13 lilies at 4 developmental stages were collected by Head Space Solid Phase Microextraction method and monoterpenes were analyzed by Gas Chromatography-Mass Spectrometry.The expression levels of LhTPS from 6 lilies at 4 developmental stages were accordingly studied by real-time quantitative PCR.Results indicated that the content of monoterpenes in the aroma components was increased first and then decreased with lily flower's opening.The main monoterpene components were ocimene and linalool for scent lilies,however,1,8-cineole and ocimene appeared more in Trumpet and Orienpet lilies.The expression levels of LhTPS in Trumpet,Orienpet and Oriental lilies were higher than that in Longiflorum and Asiatic lilies overall.The expression level of LhTPS gene was significantly correlated with the synthesis of ocimene and linalool in the blooming stage,implying that LhTPS gene played an important role in the synthesis of ocimene and linalool,and was a key gene for synthesis of floral fragrance in lilies.
Keywords:LiliumFloral fragranceGas chromatography-mass spectrometry (GC-MS)MonoterpenesLhTPS gene
Publication Date:2018-01-01
Online Publishing Date:2026-09-12(First online date of this platform, not the publication date of the document)
Pages:6( 88-92,108 )
