Effects of Levonorgestrel and Quinestrol on Reproduction of Brandt's Voles

SHI Yuhua; WANG Baishi; SUN Hong; JI Zhihong; WANG Zhenlong

doi:10.15933/j.cnki.1004-3268.2018.03.016

Abstract：We used Brandt's voles (Lasiopodomys brandtii) to study the effects of levonorgestrel (L) and quinestrol(Q)on the reproduction of rodents.A two-way ANOVA experiment,L(+/ -) × Q(+/ -),was performed on four groups of Brandt's voles.Each group included seven pairs of voles (14 individuals in total) and was given a dose of 2 mg/kg daily using the method of pouring belly in seven days. The Brandt's voles were then mated and their weight and reproduction were recorded.After thirty days,the reproductive organs were weighted and the levels of hormones in blood were measured.The results showed that Q could significantly reduce Brandt's weight,especially after 14 days of pouring belly(it was reduced by 18.16%).L and Q both significantly inhibited the reproduction of Brandt's voles.The L-Q-group had a pregnancy rate of 85.70%,while the L+Q- group only had 7.14%,and the other two groups had none.L significantly increased testis index(-2.11%—66.67%) and epididymis index (18.18%—160.00%),and Q promoted L's positive effects on the two indexes.Q significantly inhibited uterus index (-64.20%—-30.56%) and testis index (-43.16%—-3.22%),and L decreased the suppression of Q on the two indexes.There were no significant effects of Q on estradiol,testosterone,urofollitrophin and corticosterone contents.L significantly inhibited the expression of urofollitrophin(-11.83%—-55.38%), and Q increased the suppression of L. In conclusion,Q inhibits the reproduction of Brandt's voles,whereas L has a weaker suppression than Q. The combined usage of L and Q can reduce Q' s suppression.