Biocontrol Effect on Cotton Verticillium Wilt and Identification of Endophytic Bacterium HB3S-20 from Cotton
JIN Lirong
WAN Peng
HUANG Wei
Abstract：In order to further explore the biocontrol strains of cotton Verticillium wilt,an endophytic bacterium HB3S-20 from cotton was screened by greenhouse experiment,which had good biocontrol effect on cotton Verticillium wilt(62.57% on average).The results of field trial showed that the disease indexes of cotton Verticilillium wilt were decreased by watering seedling roots with the bacterium suspension of the strain HB3S-20 after transplantation, and the biocontrol effect was 54.67%. According to the morphological characteristics and phylogenetic analysis, the strain HB3S-20 was identified as Pseudomonas putida.The result of laboratory test showed that the HB3S-20 was effective in inhibiting the mycelium growth of Verticillium dahliae,and the inhibition rate was 47.30%.The CAS medium detection demonstrated that the HB3S-20 could produce siderophore. Meanwhile,HB3S-20 had growth-promoting effect on the height and biomass of cotton by pot experiment.This study indicated that the strain HB3S-20 was potentially valuable in the biological control of cotton Verticillium wilt,and had favorable practical prospects.
Keywords：Cotton Verticillium wiltEndophytic bacteriaHB3S-20Antifungal activityIdentificationBiocontrol effectGrowth-promoting effect
Publication Date：2018-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 70-75 )