Establishment of Embryogenic Suspension Cell and Plant Regeneration of Wheat Zhengmai 9023
LI Dongbing
Lü Guizhen
NIU Hongbin
YIN Jun
Abstract：In order to establish a rapid,efficient and stable genetic transformation system for wheat,rataria of wheat Zhengmai 9023 were cultured to induce calluses,and the effect of different concentrations of 2,4-D and KT in mediums on callus induction and subculture was studied.The results showed that the optimal callus induction medium was MS+ 3.0 mg/L 2,4-D,and the highest ratio of callus induction was 92.5%;the optimal medium for callus subculture was MS+ 1.0 mg/L 2,4-D+0.1 mg/L KT;the optimal medium for cell suspension was MS +1.0 mg/L 2,4-D + 0.1 mg/L KT;the optimal time for subculture of suspension system was 16 d.The mature embryos in suspension system could develop into normal plants in the basic medium.The embryogenic cell suspension could be used for genetic engineering and screening of somaclonal mutants in wheat.
Keywords：WheatZhengmai 9023Embryogenic suspension cellPlant regeneration
Publication Date：2018-03-02
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 34-37 )