Induction and Identification of Salt-tolerant Mutant of Taraxacum mongolicum Callus
LIU Yanfen
WANG Xiuping
CHEN Cuiguo
ZHAO Min
LIANG Weiling
WANG Tingting
DONG Xiuxiu
Abstract：To obtain salt-tolerant mutants of Taraxacum mongolicum,using its leaves as explants the callus of salt-tolerant mutant was screened. The results indicated that the optimal medium for callus induction and proliferation was MS+6-BA 0.5 mg/L+2,4-D 0.04 mg/L and MS+6-BA 0.4 mg/L+2, 4-D 0.02 mg/L+NAA 0.2 mg/L respectively.The salt-tolerant mutants were inoculated on medium with different salt concentrations,and screened out from the callus after 4 times of salt stress.Compared with the control without NaCl,there were different bands in the callus cultured on the medium with NaCl of 8, 10 and 12 g/L using RAPD detection.It could be coucluded that the DNA of salt tolerant callus mutated.
Keywords：Taraxacum mongolicumSalt-tolerant mutantInductionIdentification
Publication Date：2018-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 102-106 )