Effects of Two Pretreatments on Two-Dimensional Electrophoresis Maps for Total Proteins of Maize Tissues
AN Sufang
HOU Jinna
WANG Yan
LU Dandan
LI Baoquan
Abstract：In order to set up a suitable method for total protein extraction of maize tissues,the effects of acetone pretreatment and TCA acetone pretreatment on two-dimensional electrophoresis maps with maize leveas and pollens as material using PDQuest analysis.The results showed that 634 and 540 distinct pro-tein spots were separated in maize leaves by acetone pretreatment and TCA acetone pretreatment,and 410 and 320 distinct protein spots were separated in pollen.Compared with two pretreatments of maize tissues, acetone pretreatment had a small amount of trailing tail,and the protein point abundance was high,which was suitable for mass spectrometry analysis.TCA acetone pretreatment had lighter trailing tail,amount of protein point was less,which was suitable for protein difference analysis.
Keywords：PretreatmentsMaizeLeavesPollenProteinAcetoneTrichloroacetic acid(TCA)/acetone
Publication Date：2018-01-31
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 17-21 )