Cloning and Prokaryotic Expression of Transcription Factor PcMYB1 Gene from Polygonum cuspidatum
LIU Zhongyu
LEI Jian
LI Xiaoxiao
LIU Chan
QIN Jianbing
XU Feng
Abstract：In order to identify the role of MYB transcription factors in phenylpropanoid pathway of Polygonum cuspidatum,the MYB homologue gene PcMYB1 (GenBank accession No.KY495789) was amplified from the leaf of P.cuspidatum by RT-PCR and RACE methods.The full length cDNA of PcMYB1 is 1 152 bp and the open reading frame is 813 bp,encoding a protein of 270 amino acids with relative molecular mass of 30.455 ku.Sequence analysis indicated that the deduced amino acids had common characteristics of MYB transcription factors with two MYB domains of R2 and R3 at the N-terminal and a transcriptional repression domain C2 motif pdLNL[D/E]LXI[G/S] at the C-terminal.Phylogenetic tree analysis suggested that the encoded protein had the closest genetic relationship with PhMYB4 from Petunia × hybrida.The recombinant prokaryotic expression vector pET28a-PcMYB1 was constructed by inserting the DNA fragment into the prokaryotic expression vector pET-28a,and then transformed into E.coli BL21 (DE3).SDS-PAGE analysis showed that the size of expressed protein was consistent with expectation.In conclusion,the full-length sequence of PcMYB1 gene in P.cuspidatum is obtained,and the prokaryotic expression vector pET28a-PcMYB1 can be used for functional study of PcMYB1 gene.
Keywords：Polygonum cuspidatumMYB transcription factorPcMYB1Prokaryotic expressionPhenylpropanoid metabolismRACE
Publication Date：2018-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：7( 96-102 )