Synergistic Effects of Se and Ge on Contents of Main Active Components of Cordyceps militaris

WANG Jufeng; LI Huming; YANG Daode

doi:10.15933/j.cnki.1004-3268.2018.01.016

Abstract：To study the synergistic effects of Se and Ge on the contents of cordycepin,adenosine and polysaccharide of Cordyceps militaris,12 Se and Ge combination groups were prepared using 5,10,15 mg/L of Na2SeO3 and 100,200,300,400 mg/L of GeO2 and added in the basic culture medium with the conventional solid culture of Cordyceps militaris.The effects of Se and Ge on the fruitbody biomass were observed and the contents of cordycepin,adenosine and polysaccharide of Cordyceps militaris were determined by HPLC and phenol sulfuric acid.The results showed that after treatment with different concentrations of Se and Ge,all fruitbodies of sample groups grew better,and the majority was stouter and more yellow than the control group.Under the same conditions,the fresh weight and dry weight were significantly higher than the control group with the growth rate of dry weight reaching 14.50％.The contents of cordycepin,adenosine and polysaccharide in all the sample groups were higher than that of control group.When the combination concentration of Se and Ge was 10 mg/L × 300 mg/L,the content of cordycepin in fruitbody reached the highest concentration of 1.12％,which was 2.89 times higher than that of the control group.When the combination concentration of Se and Ge was 15 mg/L × 100 mg/L,the content of adenosine in fruitbody was up to 0.116％,which was the highest and 2.7 times higher than that of the control group,and when the combination concentration of Se and Ge was 10 mg/L × 200 mg/L,the content of polysaccharide reached the highest concentration of 5.72％,which was 1.93 times higher than that of the control group.Se and Ge as two kinds of trace element can significantly affect the growth of fruitbody of Cordyceps militaris and the contents of its main physiologically active substances.Simultaneous addition of Se and Ge into the basic culture medium shows obviously synergistic effect.Se and Ge can increase the biomass and the biosynthesis of cordycepin,adenosine and polysaccharide of Cordyceps militaris.