Effects of Chitooligosaccharide on the Ascorbate-Glutathione Cycle in Seedling of Wheat (Triticum aestivum L.) under PEG Stress
ZHAO Xiaoqiong
LIANG Taishuai
ZHAO Runzhu
Abstract：In order to investigate the effects of chitooligosaccharide on the ascorbate (AsA)-glutathione (GSH) cycle in seedlings of wheat(Triticum aestivum L.) under drought stress,a hydroponic experiment was performed to research the effects of foliar spraying of 100 mg/L chitooligosaccharide solution on the reactive oxygen species accumulation,membrane lipid peroxidation,key enzymes activity and metabolites contents of AsA-GSH cycle in leaves of two wheat cultivars Pubing 143 (tolerant to drought stress) and Zhengyin 1 (sensitive to drought stress) under 20％ PEG stress.The results showed that foliar spraying of 100 mg/L chitooligosaccharide solution significantly promoted the activities of ascorbate peroxidase (APX),dehydroascorbate reductase (DHAR),monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR),the contents of GSH and AsA,and the ratios of GSH/GSSG(L-glutathione oxidized) and AsA/DHA(dehydroascorbic acid),and obviously decreased the contents of superoxide anion (O2-·) and malondialdehyde(MDA),compared to PEG stress alone.These findings indicated that foliar spraying of 100 mg/L chitooligosaccharide solution could effectively enhance the wheat resistance to drought stress by maintaining the high efficiency metabolism of AsA-GSH cycle and decreasing the reactive oxygen species accumulation and membrane lipid peroxidation in leaves.
Keywords：chitooligosaccharidewheatPEG stressAsA-GSH cycle
Publication Date：2017-12-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 8-12,58 )