Cloning and Diversity Analysis of TaHKT2;2 Gene in Hexaploid Wheat
LI Mengjun
LI Yaqing
ZHANG Shichang
ZHANG Nan
SHI Zhanliang
Abstract：In order to study the roles of members of HKT gene family in wheat,TaHKT2;2 was isolated by using homologous cloning strategy and screening genomic BAC library.TaHKT2;2 genes were mapped on chromosomes 7A,7B,and 7D,named as TaHKT2;2-TA,TaHKT2;2-7B,and TaHKT2;2-7D.TaHKT2;2 and TaHKT2;1 had the same genetic structure,composed of three exons and two introns,and formed a cluster with TaHKT2;1 on phylogenetic tree of plant HKT transporters.The coding sequences of TaHKT2;2-7A,TaHKT2;2-7B,and TaHKT2;2-7D were 1 602,1 602,1 596 bp long,respectively,but TaHKT2;2-7D cDNA sequence was not isolated by RT-PCR in eight wheat varieties.The natural diversity of TaHKT2;2 genes was analyzed by cloning and sequencing from 12 wheat varieties.The results showed that TaHKT2;2-7A was found to be more diverse than TaHKT2;2-7B and TaHKT2;2-7D.Only a few bases changed in the alleles of TaHKT2;2 genes in wheat.No amino-acid natural variation lay in the P-loops of deduced protein sequences of all alleles of TaHKT'2;2 in 12 wheat varieties.The identity of coding sequences was much higher than that of 5'flanking regions of TaHKT2;2 genes.TaHKT2;2 might be selected over the course of wheat domestication and belonged to domestication gene.
Keywords：hexaploid wheatHKT gene familyTaHKT22 geneBAC librarydiversity
Publication Date：2017-12-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：7( 1-7 )