Molecular Identification and Genetic Relationship of 9 Dioscorea Species in Guizhou Province
WEI Yibing
WEI Shenghua
WANG Zhiwei
YAN Fulin
Abstract：In order to reveal the genetic relationship between 9 species of Dioscorea in Guizhou province,the molecular identification of the species was conducted using the sequence fragments of chloroplast genes matK,rbcL and psbA-trnH.The results showed that the lengths of matK,psbA-trnH and rbcL in 9 species of Dioscorea in Guizhou province were 1 076-1 144 bp,361-382 bp and 1 160-1 209 bp.When the vacancy was treated as deletion,the ratio of the mutation site in the total length was 14.3％,7.8％ and 8.7％,respectively.Phylogenetic analysis showed that 9 species of Dioscorea derived from the 31 provenances were divided into four groups.Among them,Dioscorea bulbifera L.belonged to the cluster of Sect.Opsophyton Uline.Dioscorea melanophyma Prain et Burkill and Dioscorea henryi (Prain et Burkill) C.T.Ting belonged to the cluster of Sect.Lasiophyton Uline.Dioscorea subcalva Prain et Burkill and Dioscorea nitens Prain et Burkill belonged to the cluster of Sect.Shannicorea Prainet Burkill.Dioscorea glabra Roxb.,Dioscorea cirrhosa Lour.,Dioscorea japonica Thunb.and Dioscorea opposita Thunb.belonged to the cluster of Sect.Enantiophyllum Uline.The results show that matK,rbcL and psbA-trnH have obvious guidance for systematic classification of Dioscorea.
Keywords：DioscoreaDNA barcodematKrbcLpsbA-trnH
Publication Date：2017-11-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 108-112 )