Comparison of Three Molecular Identification Methods for Fusarium graminearum sensu stricto and F.asiaticum
RU Yanyan
XIE Shuna
LI Baoye
SUN Jing
LIU Jiazhong
HAO Junjie
Abstract：Fusarium graminearum sensu stricto and F.asiaticum are major members of Fusarium graminearum species complex(FGSC) in China.In this study,to improve the identification efficiency of these two species,48 members of FGSC collected from plants showing Fusarium head blight (FHB) or maize stalk rot(MSR) were identified using three molecular identification methods.One method was amplification of partial sequences of two genes EF-1α and Tri101,and then sequencing and phylogenetic analyses by MEGA 6.06 software.The results showed that 11 isolates were clustered together with F.asiaticum and 37 isolates were clustered together with F.graminearum sensu stricto.This method was cumbersome,complex and costly,but the identification results were accurate.A second method was polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Based on the specific restriction sites of histone H3 gene,primers H3dSty Ⅰ and H3R1 were designed.11 isolates were identified as F.asiaticum and 37 isolates were F.graminearum sensu stricto.This method required lots of work and high operation process.The third method was a multiplex PCR.Based on the CYP51A gene,primers FaF/FaR and FgF/FgR were designed.11 isolates were identified as F.asiaticum and 37 isolates were F.graminearum sensu stricto.This method was simple and efficient.These results were similar,while the multiplex PCR method was more rapid,accurate and efficient.
Keywords：Fusarium graminearum species complex(FGSC)Fusarium graminearum sensu strictoFusarium asiaticummolecular identification
Publication Date：2017-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：7( 80-86 )