Bioinformatics and Functional Analysis of WRKY Transcription Factors in Interaction between Tomato and Cladosporium fulvum
WANG Qiuying
XUE Dongqi
CHEN Xiuling
LI Jingfu
WANG Aoxue
Abstract：To preliminarily define the role of WRKY transcription factors in the incompatible interaction between Cf-12 tomato and Cladosporium fulvum,we analyzed differential expression of WRKY transcription factors in the transcriptome of 4 d and 8 d time points of incompatible interaction,and then conducted phylogenetic analyses and gene silencing by virus inoculation.The results showed that 22 WRKY genes were enriched in the process of incompatible interaction,and the expression of Solyc07g047960.2 at 8 days post inoculation increased by nearly 20 times.According to phylogenetic analysis and expression analysis of WRKY CDS,the expression of some WRKY genes was not significantly different at two time points,however,in Cluster Ⅲl,7 WRKY genes showed significant up-regulated expression.After identification of WRKY gene silenced tomato plants(inoculated by TRV-WRKY),the results of Cladosporium fulvum inoculation showed that the expression of WRKY gene was increased compared with that before inoculation,and the number of hypersensitive necrotic spots on leaf was obviously lower than the control(inoculated by TRV).The conclusion was that the WRKY (Solyc07g047960.2) gene showed a significantly upregulated expression during the incompatible interaction between Cf-12 tomato and Cladosporium fulvum,and was actively involved in the resistance response to the tomato leaf mold disease.
Keywords：tomatoCladosporium fulvumWRKYdifferential expressiongene silencingresistance response
Publication Date：2017-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 74-79 )