A Spectrophotometric Method for Determining the Activity of Lipoxygenase in Sesame
CAO Shina
SUN Qiang
HUANG Jinian
GAO Jinhong
Abstract：In order to ascertain the suitable conditions for the determination of lipoxygenase activity in sesame,the activity of lipoxygenase in sesame was determined by ultraviolet spectrophotometry and the measured conditions were discussed.The results showed that the optimal reaction conditions were as follows:2.95 mL of phosphate buffer (0.05 mol/L,pH 6.2),20 μL of sodium linoleate (10 mmol/L) as the substrate,and 50 μL of enzyme solution.Blend them quickly and immediately observe the change of OD value at 234 nm UV light.The optimal reaction time was 0-1.5 min.The enzyme activity was calculated using the equation according to the absorbance value.In addition,the activity of lipoxygenase in sesame decreased with the elongation of the storage time of the enzyme solution,which should be kept at 4 ℃ and measured within 1 h.When the added volume of the enzyme solution was between 10-50 μL,the enzymatic activity (y) was in direct proportion to the enzyme volume (x),y =0.084 6x + 5.858,and the linear relationship was good (R2 =0.999 8).The results of the precision test showed that the RSD was 0.027％,and the established method was convenient,stable and suitable for wide application.
Keywords：sesamelipoxygenaseactivity determinationUV spectrophotometry
Publication Date：2017-11-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 48-51 )