Cloning and Functional Analysis of JcWRI1 Promoter of Jatropha curcas L.
TANG Yuehui
LIU Kun
ZHAO Junwei
XU Kedong
ZHANG Ju
BAO Xinxin
ZHANG Yi
LI Xiaoli
QI Jing
YU Deshui
WANG Jian
LI Chengwei
Abstract：WRI1 plays an important role in controlling the conversion course from carbohydrates to lipids.We analyzed the expression pattern of the JcWRI1 in different tissues in Jatropha curcas L.by qRT-PCR.The result showed that JcWRI1 transcripts were predominantly expressed in seeds.On the other hand,the promoter of JcWRI1 was obtained from Jatropha curcas L.in this paper.Sequence analysis indicated that the promoter included TATA-box,CAAT-box,and a number of cis-acting elements involved in abscisic acid response,light response,stress response,endosperm-specific expression,respectively.The JcWRI1 promoter was fused with GUS reporter gene and transferred into Oryza sativa L..Histochemical staining of different organs of the transgenic plants showed that a very lowly GUS reporter gene expression in root,stem,leaf and higher expression in seed.The results indicated that the promoter functioned in the seed and regulated by multiple factors.
Keywords：Jatropha curcas L.promotertranscription factorJcWRI1 gene
Publication Date：2017-10-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 110-115 )