Bioinformatics Analysis of Iron Uptake Key Gene in the Root of Pyrus betulifolia and the Effect of Iron Deficiency on Its Expression
GUO Xianping
WU Zhongying
WANG Dongsheng
ZHANG Sipu
NIU Jiajia
Abstract：To explore the effect of iron deficiency on iron uptake key gene expression in the root of Pyrus betulifolia,the amino acid sequences of ferric reduction oxidase gene(PbFRO2) and iron-regulated transporter gene(PbIRT1) of Pyrus betulifolia were separately compared with other plants,phylogenetic analysis was used to examine the evolutionary relationships,and the effects of iron deficiency on the PbFRO2 and PbIRT1 gene expression and Fe and zinc contents in the root of Pyrus betulifolia were studied by using the modified Hoagland nutrient solution for culture.The results showed that PbFRO2 protein had reductase features,and PbIRT1 protein was one of the members of the ZIP family.The PbFRO2 and PbIRT1 gene expression showed a trend of increase first and then decrease under iron deficiency for 9 days,and the expression level reached its highest point on the 3rd day,with a significant difference from the control.Fe content in root was reduced by 77.46％,and the zinc content was increased by 1 139.40％ on the 30th day under iron deficiency.In conclusion,the increase of iron uptake key gene expression in the root of Pyrus betulifolia can be induced under iron deficiency stress,which further promotes the activity of divalent metal ion transporters.
Keywords：Pyrus betulifoliairon deficiencyiron uptakeferric reduction oxidase geneiron-regulated transporter gene
Publication Date：2017-08-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 96-101 )