Isolation, Identification and Phosphorolysis Characteristics of High-efficient Phosphate-solubilizing Bacterium XMT-5 under Oligotrophic Conditions
LI Haifeng
ZHANG Yueyang
CAO Jian
QU Jianhang
TIAN Hailong
Abstract：In order to obtain strains which could adapt more oligotrophic natural environmental conditions,Monkina medium diluted by 4 times was used to isolate high-efficient phosphate-solubilizing bacteria,and the bacteria were identified,the growth condition and phosphorolysis mechanism were studied.The results showed that nine phosphate-solubilizing strains were screened out,and the high-efficient phosphate-solubilizing strain XMT-5 was determined through the quantitative determination of phosphate-solubilizing quantity,which could solubilizing phosphate 195.63 mg/L.According to the colony morphology,physiological and biochemical characteristics and 16S rRNA gene sequence analysis,strain XMT-5 was identified as Rhizobium sp.Under the oligotrophic condition,strain XMT-5 could secrete citric acid,tartaric acid and acetic acid,so lead to a drop of pH value.Strain XMT-5 could grow under the oligotrophic condition with 4-42 ℃,pH value of 4-10 and NaCl of 0-80 g/L.The strain had strong adaptability to the environment,and good application prospect.
Keywords：oligotrophicphosphate-solubilizing bacteriaphosphorolysis characteristicsbiological phosphorus fertilizer
Publication Date：2017-08-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 67-71 )