Screening of Interacted Proteins of TaMBD2 in Wheat by Yeast Two-hybrid System
LING Na
YANG Yanmin
HOU Jiangtao
MENG Fanrong
Abstract：In order to understand more regulatory functions of methyl-binding domain protein TaMBD2 during the growth and development in wheat,bait vector pGBKT7-TaMBD2 was constructed with the full length cDNA of TaMBD2 gene as template,and the interacted proteins of TaMBD2 from wheat cDNA library were screened by yeast two-hybrid system.The result showed that a total of 91 blue clones were screened,which were analyzed through PCR and homology analysis using the BLAST in NCBI,and eight possible interacted proteins of TaMBD2 were obtained,which were nucleoside diphosphate kinase (NDPK),epsin N-terminal homology domain-containing protein,seven in absentia family protein,Agenet domain-containing protein,zinc finger C2H2 type family protein,phosphate kinase and two hypothetical proteins.The most likely interacted protein of TaMBD2 was NDPK.These candidate proteins were related to mediate cell signal transduction,stress resistance,energy metabolism and protein transport,etc.Among them,the proteins involved in resistance to plant stress were the main interacted protein,the proportion was 54.9％;the proportion of proteins participated in protein transport was 25.3％,and the proportion of the rest interacted proteins was smaller.Therefore,TaMBD2 may mainly participate in response and regulation to abiotic stress,such as drought,low temperature and high salt.
Keywords：wheatyeast two-hybridTaMBD2interacted proteins
Publication Date：2017-08-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 7-12 )