QTL Mapping of Seed Germination Traits under Different Temperature Conditions Using Recombinant Inbred Lines of Maize

HAN Zanping; CHEN Yanhui; LIU Haiying; ZHAO Ruifang; GUO Shulei

doi:10.15933/j.cnki.1004-3268.2017.07.002

Abstract：Two recombinant inbred lines (RILs) populations which were obtained from Yu 537A × Shen 137 and Yu 82 × Shen 137 and contained 420 family lines were used as materials,two high-density molecular linkage maps were constructed using single nucleotide polymorphism (SNP) molecular marker technology.Through composite interval mapping method,the QTLs of seed vigor-related morphological and physiological traits were detected,including percentage of germination (GP),germination energy (GE),germination index (GI),vigor index (VI),seedling length (SL),seedling dry weight (SDW),root dry weight(RDW).Sixty-four QTLs for seed vigor-related traits were detected in the two connected RILs population in normal and low temperature conditions.The results showed that twenty-four QTLs were found in the population based on Yu 82 × Shen 137,and were located on chromosomes 1,2,3,4,5,6,7 and 10,with the explanation of phenotypic variation of a single QTL from 5.61％ to 11.01％.Others fourty QTLs were found in the population based on Yu 537A × Shen 137,and located on all chromosomes except 2,with the explanation of phenotypic variation of a single QTL from 5.39％ to 11.92％.Six mQTLs were identified on chromosomes 3,5,7 and 10 by meta-analysis,and twenty-five initial QTLs were integrated,the consolidation ratio was 39.1％.Each mQTL contained 4.17 QTLs on average,involved in the regulation of two to four traits,including VI,SDW,RDW,GE,mQTL3-2 contained 7 QTLs.Thirty-five candidate genes were located in 6 mQTLs corresponding SNP marker distribution ranges,which involved in the regulation of seed germination,plant development,growth metabolic pathways,signal transduction,withstanding adversity and so forth.