Construction of Chlorophyll Degradation Engineering Bacteria
YANG Zongcan
ZHANG Zhan
PENG Yufu
HOU Pei
NIE Cong
MA Yuping
Abstract：For the specific and efficient degradation of chlorophyll in tobacco leaves,the chlorophyllase gene AtCLH1 containing a full coding region of 975 bp was cloned from Arabidopsis thaliana.AtCLH1 gene was cloned into vector pET28a,and the vector was then transformed into E.coli BL21 cells,finally the recombinant engineering bacterium was obtained.After culturing under 30 ℃ for 22 h,the protein AtCLH1 could express well with 0.5 mmol/L IPTG(isopropy-β-D-thiogaIactopyranoside) as inducer.Sodium dodecyl sulfate(SDS) fingerprint results showed that the molecular mass of AtCLH1 was 35 ku.The activity of AtCLH1 was 24.9 U/mL,which could degrade the chlorophyll in tobacco extract and had a good application prospect in improving the quality of low quality tobacco.
Keywords：tobacco leafengineering bacteriachlorophyllase generecombinant proteinchlorophyll degradation
Publication Date：2017-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 39-42 )