Detection of Residual Fenitrothion in Vegetable Samples by BIAcore Based on Single Chain Antibody
LIU Shan
LUO Yihui
Abstract：For establishing a method to fast measure residual fenitrothion in vegetables based on single chain antibody,the affinity and specificity of single chain variable fragments (ScFvs) against fenitrothion selected by ribosome display in our preliminary study were tested,and then a method for detection of residual fenitrothion in real vegetable samples by BIAcore X system based on the ScFvs was established.The results showed that three ScFvs of high affinity were obtained,the equilibrium dissociation constants with fenitrothion were 4.56 x 10-10,1.42 x 10-9,2.66 x 10-10 mol/L,respectively,and the cross-reactivity with other organophosphorus pesticides was below 0.1％ except for parathion-methyl(≤2.8％),which indicated that the ScFvs had a high specificity for fenitrothion.Recoveries of fenitrothion from fortified vegetables were in the range of 95.8％-102.2％ and the relative standard deviation(RSD) was below 2.13％.The results above indicated that this method was reliable,and could be used for fast measuring residual fenitrothion in real vegetable samples.
Keywords：fenitrothiondetection of organophosphorussingle chain antibodyBIAcore
Publication Date：2017-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 95-99 )