Biodegradation of Chlorpyrifos-methyl and Its Hydrolysis Product 3,5,6-Trichloro-2-pyridinol by Different Degrading Bacteria
LI Xiaolou
Abstract：To understand the degradation characteristics and mechanism of chlorpyrifos-methyl by microorganisms,First,different chlorpyrifos-methyl degrading bacteria were isolated from soil,and then,their degradation characteristics were tested and analyzed,including degradation efficiency on chlorpyrifosmethyl,variation of intermediate (3,5,6-trichloro-2-pyridinol,TCP) concentration in the degradation process,phosphatase activity,degradation ability on TCP and 6 organophosphorus pesticides.Two chlorpyrifos-methyl degrading bacteria were isolated,which were identified as Bacillus licheniformis ZL-7 and Pseudomonasfluorescens ZHLXL-2,and their degradation rates on chlorpyrifos-methyl were 90.6％ and 99.4％ respectively in five days.TCP was found in the degradation process of chlorpyrifos-methyl by the strain ZL-7,but not by the strain ZHLXL-2.The strain ZHLXL-2 could degrade TCP,and the degradation rate reached 91.0％ at 48 h,while the strain ZL-7 could not degrade TCP.Both strains could degrade 6 kinds of organophosphorus pesticides.The 10 d degradation rate of 6 organophosphorus pesticides by the strain ZL-7 was 92.1％-99.8％,higher than the degradation rate by the strain ZHLXL-2 (89.2％-93.4％).In addition,the phosphatase activity of strain ZL-7 was significantly higher than that of strain ZHLXL-2.These results showed that the phosphatase activity of the two strains was positively correlated with their ability to degrade organophosphorus pesticides.The strain ZHLXL-2 could degrade chlorpyrifosmethyl more quickly because of its ability to degrade TCP effectively.
Keywords：Bacillus licheniformisPseudomonas fluorescensorganophosphorus pesticidechlorpyrifosmethyl356-trichloro-2-pyridinoldegradation
Publication Date：2017-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 84-89 )