Analysis of Genetic Diversity of Populus from Three Different Factions by SSR Markers
HAN Zhixiao
ZHANG Jun
ZUO Lihui
REN Yachao
Abstract：Thirteen pairs of SSR primers which met the requirements were screened from 28 pairs of poplar basic core primers.The PCR amplification of 32 poplar fines from Sect.Leuce Duby,Sect.Aigeiros Duby and Sect.Tacamahaca Spach was done by use of these primers,and the genetic diversity and genetic relationship of these samples were analyzed at the same time.The results showed that 58 DNA bands were obtained,and polymorphic bands accounted for 100.00％ of amplification bands.The calculating results showed that the genetic distance of 32 poplar lines ranged between 0.056 and 0.741,which indicated that high genetic diversity existed among the samples.The result of cluster analysis showed that the samples could be divided into three clusters,namely Sect.Leuce Duby,Sect.Aigeiros Duby and Sect.Tacamahaca Spach.And the clustering results were consistent with the results of traditional taxonomy,which showed the reliability of SSR molecular marker technology in identification of different species of Populus.
Keywords：PopulusSSRgenetic diversity
Publication Date：2017-04-02
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 99-103 )