Enzymatic Properties of Five Different Fusarium Species

WANG Guiqing; MA Di

doi:10.15933/j.cnki.1004-3268.2017.04.016

Abstract：The enzymatic analysis of five Fusarium species causing root rot disease of adult Chinese scholar tree was carried out by colorimetry and electrophoresis,to provide a theoretical basis for classification and study on physiological differentiation of Fusarium.The results showed that the assay results of soluble protein content and enzymatic activity of four protective enzymes (EST,SOD,PPO and POD) were consistent with their electrophoresis results of the five Fusarium species.For example,the SOD enzyme activities of five Fusarium species were very high,in the range of 2 263.09-6 134.42 U/mg,meanwhile,their electrophoretic patterns were clear,and the color of the spectra was darker and brighter,which indicated that the activity of protective enzymes could be semi-quantitatively determined by electrophoresis.The results also showed that a significant diversity of Fusarium species was found in terms of spectrum change of soluble protein and isozyme including EST,SOD and PPO among those strains,and the obvious difference in the number of bands and brightness and color of bands with the same Rf value was also detected,so there were both common bands and specific bands.The common bands of EST isozyme patterns of five Fusarium species accounted for 23.5 ％,and the specific bands accounted for 76.5％.This indicates that the five pathogens are all members of the genus Fusarium,but respectively belong to different Fusarium species.