Construction of Tn5 Mutant Library of Ralstonia solanacearum and Analysis of Mutation Sites
LI Xiaojie
LI Shujun
LI Chengjun
CHEN Yuguo
WANG Haitao
QIU Rui
HU Yajing
Abstract：In order to explore the pathogenic genes from the whole genome range of Ralstonia solanacearum,this study constructed a Ez-Tn5 transposon insertion mutant library of R.solanacearum TX-LLJ14-3 by electroporation method.The library contained 12 000 mutants.There were 216 non or weak pathogenic mutant strains by the pathogenic detection on tobacco.Tn5 flanking sequences of 15 non pathogenic mutant strains were amplified by TAIL-PCR,and simultaneously,the insertion sites were analyzed.The results showed that the insertion sites of the 15 mutant strains were located at genes with different functions such as nucleotide hydrolases,glycosyltransferases,transposase and synthase.When they were disrupted or destroyed,the expression or secretion of virulence related substances might be inhibited,or tobacco resistance to pathogen be induced,thus showing no pathogenic characteristics.
Keywords：Ralstonia solanacearumTn5 insertion mutationnon pathogenic strainTAIL-PCRmutation site
Publication Date：2017-04-02
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 80-83,94 )