Diversity, Antimicrobial Activity of Endophytic Fungi from Camellia oleifera

ZHANG Ting; ZUO Xuezhi; XU Yan

doi:10.15933/j.cnki.1004-3268.2017.04.013

Abstract：To understand the classification,distribution feature and applied potential of endophytic fungi from C.oleifera,we collected samples from five plantingregions in Hubei province in different seasons.Endophytic fungi were separated from these C.oleifera for the diversity and antimicrobial activity analysis.In this study,1 612 stains of ebdophytic fungi were isolated,which were categorized into 24 genera by morphological analysis.Sixty-six strains with no reproductive features were classified as sterile mycelia.The dominant groups were Pencillium,Alternaria and Colletotrichum,accounting for 21.09％,19.11％and 14.70％ of isolated strains respectively.Diversity analysis of isolated strains in different regions indicates that endophytic fungi from Huanggang showed highest Shannon-wiener diversity index (2.44);the highest indexes were in stem(2.52) and in autumn(2.58) in different tissues and different seasons respectively.In general,the populations of endophytic fungi from C.oleifera were stable,the composition of endophytic fungi in the samples from different regions or different tissues was similar.Antimicrobial assay indicated that fermented extract in 17 of 24 chosen strains had antimicrobial activity,3 strains exhibited greater activity than the others,which belonged to Pencillium,Alternaria and Phyllosticta,respectively.Our results demonstrated that endophytic fungi from C.oleifera had great antimicrobial activity,and were worth further studying.