Soluble Expression and Immuneoreactivity Analysis of FMDV VP0 and VP1 Protein
Abstract：To obtain efficient soluble expressive VP0 and VP1 protein of O-type foot-and-mouth disease virus ( FMDV) . We optimized VP0 and VP1 gene according to the preference codon usage of E. Coli. The FMDV structural protein VP0 and VP1 genes were synthesized and cloned into pE-SUMO vector. These two recombinant plasmids,SUMO-VP0 and SUMO-VP1 were transformed into E. coli BL21(DE3) compe-tent cells. Through optimizing the inducing temperature,time and the concentration of IPTG,we got that the optimized expression conditions of SUMO-VP1 was induced by 0. 1 mmol/L IPTG,and induced at 20 ℃ for 8 h . The best inducing conditions of SUMO-VP0 was induced by 0. 1 mmol/L IPTG,and ex-pressed 12 h at 37℃. SDS-PAGE and Western blot analysis showed that the soluble protein could be identified by standard positive serum of FMDV,which confirmed that the fusion protein had good responsiveness.
Keywords：FMDVstructural protein VP0 and VP1soluble protein expressionSUMO tag
Publication Date：2017-01-31
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 105-110 )