Comparative Study on Antioxidant Activity of Alcohol Extracts from Different Millet Cultivars

WANG Lingyun; SONG Xiaoqing; WANG Xiaoming; FAN Guangyu; ZHAO Zhihai; ZHAO Yan; LIU Yinghui

doi:10.15933/j.cnki.1004-3268.2017.02.006

Abstract：In order to study the antioxidant activity of millet,5 millet cultivars were used to determine the content of antioxidative substance,the antioxidant capacity of alcohol extract from different millet cultivars were compared, and the relationship between the antioxidant capacity and the content of antioxidative substance was analyzed. The results showed that the content of the total phenol, flavonoid and alpha tocopherol ranged from 369. 9 to 793. 1 mg/kg, 162. 1 to 459. 3 mg/kg and 7. 1 to 17. 1 mg/kg, respectively. The IC50 of DPPH free radical scavenging, OH free radical scavenging and the power of reducing ranged from 0. 09 to 0. 24 mg/mL,0. 41 to 0. 74 mg/mL and 0. 98 to 2. 35 mg/mL respectively. Zhangzagu No. 9 had the lowest contents of polyphenols,alpha tocopherol,the highest flavonoid content, and the DPPH free radical scavenging capacity was the highest in all test cultivars. 8311 had the highest total polyphenols and alpha tocopherol contents,the lowest flavonoid content,the strongest OH free radical scavenging capacity. Zhangzagu No. 3 had the highest power of reducing. The correlation analysis showed that the DPPH free radical scavenging capacity was positively correlated with the contents of total polyphenol and alpha tocopherol, negatively correlation with flavonoid content. The OH free radical scavenging capacity was negatively correlated with the contents of total polyphenol and alpha tocopherol, postively correlated with flavonoid content. The reducing power was negatively correlated with alpha tocopherol content.