Functional Markers Development of Restore Gene Rf6 in Rice and Screening of Rf6 in Micro-core Collections
Abstract：Rf6 is a new rice restoring gene of HL type,in order to select more restoring lines with Rf6 for increasing restoring lines to hybrid more combinations,the functional marker of Rf6 was developed according to the difference of sequence of Rf6 and its allele,and this marker was used to screen lines with Rf6 among Chinese rice micro-core collections. The results indicated that the marker designed in both sides of the inserted 324 bp fragment(there was a 324 bp inserting fragment between Rf6 and its allele)could generate clear andnon-background production, and this marker ( forward sequence 5′-ATGACAAGAGGACCAGCGATGCAATGG-3′, reverse sequence 5′-ATTCTTGCAGAGATAGACCATGAGCGTG-3′) could identify Rf6 germplasm. Twenty-two lines including Rf6 restoring gene were selected in 197 Chinese rice micro-core collections using the above marker,and these materials would broaden restoring lines of HL hybrid rice.
Keywords：HL type ricerestoring gene Rf6micro-core collections
Publication Date：2017-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 12-15 )