Genetic Relationship of Bolete Mushrooms by FTIR Combined with Chemometrics

YAO Sen; ZHANG Ji; LI Jieqing; WANG Yuanzhong; LIU Honggao

doi:10.15933/j.cnki.1004-3268.2017.01.020

Abstract：In order to establish a new method for identifying the genetic relationships and provide theoretical basis for artificial cultivation of bolete mushrooms,fourier transform infrared (FTIR) spectroscopy combined with Chemometrics was used to study the genetic relationships among different species of bolete mushrooms samples.A total of 72 infrared spectra of 12 different species of boletes were collected and processed by using second derivative(2D),standard normal variate(SNV) and wavelet compression(WC).Then the processed spectra were used to establish a discrimination model based on partial least squares discriminant analysis(PLS-DA).Hierarchical cluster analysis was performed by the method of between-groups linkage and Euclidean distance according to the eight principal components which were obtained by PLS-DA and the results of HCA were displayed as dendrograms.The results showed that all of the original infrared spectra were similar.The common absorption peaks were mainly assigned to the functional groups of O-H,C =O,C-O-H,C =O and C-C of protein,polysaccharide,fibres and amino acid.Comparing the discrimination results of samples with spectra processed by different methods,better discrimination could be accomplished when the spectra were processed by 2D + WC.From the clustering results of different species,Boletus sinicus and Leccinum extremiorientale had the closest relationship and these two species were close to Heimioporus retisporus.B.obscureumbrinus and B.edulis had a close relationship while the relationship between B.speciosus and B.calopus was also close and both of these two mushrooms were similar to B.umbriniporus.The results suggest that FTIR can be used in the study of genetic relationship of bolete mushrooms,which may provide a new method for studying the genetic relationship of wild edible mushrooms.