A Rapid Detection Method for Gibberella saubinetii
ZHANG Lin
ZHANG Mengya
KANG Jian
YAN Hongfei
LIU Daqun
Abstract：To establish a rapid,simple and exact method for detection of Gibberella saubinetii in China,this study extracted DNA by using diseased ear directly,and combined with specific molecular marker for the rapid detection of Gibberella saubinetii.The genomic DNA of Gibberella saubinetii could be rapidly extracted and successfully detected by specific molecular marker with this detection method.Through the detection and verification of 41 samples from four provinces(Hebei,Henan,Jiangsu and Anhui provinces),the results showed that 18 samples from Anhui and Jiangsu were Fusarium asiaticum,12 samples from Hebei were Fusarium graminearum,and two pathogenic strains existed in the 11 samples from Henan.The detection result consisted with the situation of regional distribution of two pathogenic strains.The method can not only achieve the effect of traditional methods(DNA is extracted by CTAB method),but also have the advantages of rapidness and simplicity.It can be used in the study of population structure and regional distribution of Gibberella saubinetii.
Keywords：wheatGibberella saubinetiirapid detectionFusarium graminearumFusarium asiaticum
Publication Date：2017-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 88-92 )