Cloning and Bioinformatic Analysis of α-gliadin Genes in Zhengmai 366
LI Wenxu
CHANG Yang
YANG Pan
WANG Meifang
HE Shenglian
WU Zhengqing
LEI Zhensheng
CHAO Yueen
Abstract：To reveal the composition of α-gliadin protein in high quality and strong gluten wheat variety Zhengmai 366,the PCR amplification were applied to clone α-gliadin genes in Zhengmai 366 with degenerate primers.In total,13 nucleotide sequences were amplified,while the deduced amino acid sequences of nine encoded complete open reading frames.Further analysis showed that the cloned genes (named as ZM366-1-ZM366-9 respectively) had the typical characteristics of α-gliadin.According to the number of T cell stimulatory toxic epitopes and the characteristics of polyglutamine domain,ZM366-1 and ZM366-2,ZM366-3 and ZM366-4 and ZM366-5-ZM366-9 were localized to the 6A,6D and 6B chromosome respectively.Secondary structure prediction showed that all the nine α-gliadin cloned in the present study only contained α-helix structure,and the content of α-helix was significantly higher in α-gliadin of B genome than that of others.Phylogenetic tree analysis showed that the cloned α-gliadin of present study had obvious genomic specificity.
Keywords：wheatα-gliadincloningbioinformatic analysis
Publication Date：2017-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：7( 13-18,25 )