Isolation and Identification of Verticillium dahliae on Chinese Redbud in Henan
ZHAO Yang
MI Jianhua
LI Honglian
WEN Caiyi
WANG Min
Abstract：The leaves of Chinese redbud in Zhengzhou Chinese Redbud Mountain Park turned yellow,and the vascular turned brown,with seriously disease plants dead. In order to determine the cause of death, Chinese redbud with the Verticillium wilt symptom was collected and two isolates of Vcg1 and Vcg4 were isolated from infected Chinese redbud stems. The colonies of isolates on PDA showed gray white first, later formed microsclerotia,and the conidiophores were verticillate.The rDNA-ITS of Vcg1 and Vcg4 was amplified by PCR and sequenced. The sequence blast resulted in the highest sequence homology with V. dahliae Kleb. . The pathogenicity of isolates was tested by dipping Chinese redbud seedlings roots in conidial suspensions. The pathogens could infect Chinese redbud,and made the vascular bundle brown, seriously causing plant wilted and dead. The results showed that the pathogen causing Chinese redbud wilt in Zhengzhou Chinese Redbud Mountain Park was V. dahliae Kleb. .
Keywords：Chinese redbudVerticillium wiltVerticillium dahliaerDNA-ITSblast
Publication Date：2016-09-30
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 89-92 )