Genomic Characterization of DNA-A and Associated Satellite DNA Molecule of Tomato Yellow Leaf Curl Virus Infecting Tomato in Henan
WANG Haoquan
JIU Min?
WANG Lunji
QIU Zhijun
HUANG Shi
ZHANG Min
Abstract：Four virus isolates including HNCG1,HNCG2,HNCG3 and HNCG4 were obtained from tomato plants showing leaf curl symptom in Changge city,Henan province. In order to clear the pathogens and find out the genetic relationship between each other,PCR was adopted firstly to detect the tomato samples using degenerate primers for geminiviruses. A 500 bp fragment was obtained from all the samples, and the nucleotide sequence identity between the four isolates reached 99%. The complete DNA-A sequence of HNCG4 was determined to be 2781 bp. Comparisons showed that the DNA-A of HNCG4 had the highest sequence identity(99%) with that of tomato yellow leaf curl virus(TYLCV) isolates HNSQ1,HNNY1, HNLY1,HNQX from Henan, and TYLCV LF, TYLCV BJ3 isolated from adjacent Hebei and Beijing, respectively. PCR analysis demonstrated that isolates HNCG1 and HNCG4 were associated with DNAβcomponent of 1347 bp, but isolates HNCG2 and HNCG3 were not. Sequence comparisons revealed that DNAβ of HNCG1 and HNCG4 was 100% similar to each other, and they shared the highest sequence identity(88%) with that of TYLCV Vietnam isolate DX2. In conclusion,leaf curl symptom of tomato in Changge city was caused by TYLCV,and partial isolates of the virus were associated with DNAβmolecule.
Keywords：tomato yellow leaf curl virusDNA-ADNAβgeminivirustomato
Publication Date：2016-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 71-75,84 )