Genetic Diversity Analysis of Domestic and Foreign Alfalfa Germplasm with SCoT Molecular Markers

WANG Jiansheng; HOU Guiling; CHENG Liping; XIE Yongfeng

Abstract：In order to identify the genetic relationship of the new introduced alfalfa germplasm and apply it to alfalfa breeding,the genetic diversity of 19 domestic and foreign alfalfa germplasm was analyzed using SCoT markers in this study. A total of 78 effective loci were obtained for all alfalfa germplasms using 14 SCoT primers,of which 72 loci were polymorphic,and the percentage of polymorphism loci was 92. 31%. The mean value of effective number of alleles( Ne) ,Nei' s gene diversity( H) ,Shannon' s information in-dex(I) and polymorphism information content(PIC) was 1. 53,0. 31,0. 46 and 0. 31,respectively. The genetic similarity coefficient of all alfalfa germplasm ranged from 0. 385 to 0. 846,and the mean of genetic similarity coefficient was 0. 657,which suggested that the studied alfalfa germplasms have the plenty ge-netic diversity. The results of cluster analysis and principal component analysis showed that all alfalfa germplasm could be divided into two groups. The first group was constituted by 16 alfalfa germplasms, which account for 84. 21% of the total alfalfa germplasms. The second group contained three alfalfa germ-plasms only. The comprehensive analysis showed that the new introduced 19 alfalfa germplasm have the plenty diversity and it have the better value in alfalfa breeding in the future.