Cloning and Function Analysis of Hydroxycinnamoyl-CoA Quinate/Shikimate Hydroxycinnamoyl Transferase Gene in Camellia sinensis
WEI Li
Abstract：A hydroxycinnamoyl-CoA quinate/shikimate hydroxycinnamoyl transferase cDNA( CsHCT) was cloned from Camellia sinensis. CsHCT encoded a protein of 430 amino acids,which molecular weight was speculated 48. 6 ku. Sequencing results showed CsHCT contained two conserved acyltransferase domain HXXXD and DFGWG, and the homology between CsHCT and other plants HCTs ranged from 58%—82%. Phylogenetic analysis showed that the homology between CsHCT and HCT from coffee was the highest, composing a group with other plants HCTs. CsHCT protein was purified after heterologous expression in E. coli. Catalytic activity analysis showed that the recombinant CsHCT was capable of catalyzing quinic acid and shikimic acid to generate coumaroyl quinic and coumaroy shikimate;but CsHCT could not catalyze quinic acid and caffeoyl-CoA to generate CGA.
Keywords：Camellia sinensischlorogenic acidhydroxycinnamoyl-CoA quinate/shikimate hydroxycin-namoyl transferase
Publication Date：2016-09-30
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：6( 35-39,49 )