Study on Improvement of Differentiation Rate of Green Plantlets in Anther Culture of Indica Restorer Lines
LIU Kai
TANG Hongsheng
YAN Guohong
SUN Mingfa
Abstract：As MS medium was control,the effects of M8 and N6 mediums on anther callus induction rate of indica restorer lines were studied,as well as the differentiation rate of indica restorer lines were studied with different concentrations of sorbitol and activated carbon added in medium. The results showed that four indica restorer lines in M8 medium had the highest rate of callus,ranging from 20. 97% to 30. 63%, significantly higher than that in MS medium;while in the N6 medium,the rate of callus was 10. 77%—13. 70%,only Yanhui 559 and Yanhui 888 were significantly higher than that in MS medium. With the increase of concentration of sorbitol,the green plantlets differentiation rate of four indica restorer lines in the M8 differentiation medium increased, reached the highest value with 30 g/L sorbitol, ranging from 30. 57% to 36. 77%, followed by 20 g/L sorbitol, both significantly higher than the treatment without sorbitol. With the increase of concentration of activated carbon,the green plantlets differentiation rate of four indica restorer lines in M8 differentiation medium increased first and then decreased, reached the highest value with 1 g/L,ranging from 21. 38% to 27. 82%,followed by 2 g/L,both significantly higher than the treatment without activated carbon.
Keywords：indicarestorer linesanthertissue culture
Publication Date：2016-09-30
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：3( 32-34 )