Cloning and Expression Characteristics of BnTIR1 from Brassica napus L.
CHEN Fan
XIE Lingli
YUAN Chengfei
XU Benbo
Abstract：Transport inhibitor response 1 (TIR1),as an auxin receptor,plays an key role in response to auxin signals. An TIR1 gene,denoted as BnTIR1,was cloned by the rapid amplification of cDNA ends (RACE) from Zhongshuang No. 9. The genomic sequence length of BnTIR1 was 2502 bp,which con-tained two introns. The cDNA sequence length of BnTIR1 was of 2355 bp,which had a 1824 bp ORF en-coding a polypeptide of 607 amino acids. The prediction results indicated that the BnTIR1 protein con-tained conserved AMN1 domain. qRT-PCR results showed the BnTIR1 gene were expressed in roots, stems,shoots,eaves,flowers and siliques,but the transcription level were different among the six tissues, which was the highest in leaves,lowest in flowers;BnTIR1 were induced by drought and ABA,which indi-cated that BnTIR1 could involve in responding to stress.
Keywords：Brassica napus L.cloningtransport inhibitor response 1ABA
Publication Date：2016-04-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 49-53 )