Molecular Detection of Garlic Virus Disease in Henan Province
SUN Xinyan
SHI Yajuan
WANG Zhenyue
YAN Zhaoling
SUN Hu
SHI Yan
Abstract：To determine the pathogen causing garlic dwarf, garlic samples showing dwarf symptom were collected for RT-PCR analysis using five primer pairs. The results showed that onion yellow dwarf virus ( OYDV) ,leek yellow stripe virus ( LYSV) and shallot latent virus ( SLV) were detected in the sample, while garlic common latent virus ( GarCLV ) and shallot X virus ( allexiviruses ) were not found. According to the sequence and genetic evolution analysis,OYDV and SLV Henan isolates both belonged to a separate branch with the nucleotide homology with other isolates between 94 . 8% and 96 . 9% for OYDV and between 86. 5% and 88. 5% for SLV. LYSV Henan isolate was closely related to Mexio isolate with the homology of 99. 4%. This test simultaneously detected three kinds of viruses, indicating that garlic virus disease in Henan was induced by complex infection of a variety of viruses.
Keywords：garlic virus diseasemolecular detectionsequence analysisHenan province
Publication Date：2016-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 102-105 )