Harm-elimination Effects of Seed Treatment with Different Agents on Bacterial Canker of Tomato

WANG Xiqiao; ZUO Jiani; YOU Jia; LI Chunlei; WANG Junping?

doi:10.15933/j.cnki.1004-3268.2016.03.018

Abstract：The tomato seeds infected with bacterial canker were treated by different concentrations of trisodium phosphate, calcium hypochlorite, tsunami, formaldehyde, potassium permanganate, hydrochloric acid,and sodium dichloroisocyanurate solutions for different time,to screen out the optimum concentration and optimum processing time of each agent. The conventional PCR method was used to detect the effectiveness of harm-elimination treatment to inoculated seeds, and plate culture method was used to detect the inhibition effect of agents on the pathogen of bacterial canker. The results showed that the most appropriate treatment conditions for each agent were as follows:8% trisodium phosphate treated seeds for 30 min,1% calcium hypochlorite treated seeds for 20 min,0. 4% tsunami treated seeds for 20 min,100-fold dilution of formaldehyde treated seeds for 20 min,hydrochloric acid with pH value of 1. 5 treated seeds for 10 min, 1% potassium permanganate treated seeds for 30 min, 700-fold dilution of sodium dichloroisocyanurate treated seeds for 40 min. The germination rate of treated seeds was tested by paper bed,and the results showed that the agents had no significant influence on tomato seed germination. Integrating their own characteristics of seven agents,the optimum treatment conditions and the impact on germination rate, sodium dichloroisocyanurate is the best agent to eliminate the pathogen of tomato bacterial canker by seed treatment.