Genome-wide Transcriptome Analysis of Yeast SOD1 Gene Deletion Mutant in Response to Calcofluor White Stress
ZHANG Xiaohua
LIU Xiangyong
BIAN Weihua
XU Yanyan
XU Cong
Abstract：This paper investigated the effect of SOD1 gene deletion on the genome-wide transcriptional re-sponse to fungal cell wall-perturbing agent Calcofluor white( CFW) in Saccharomyces cerevisiae using DNA microarray,to provide a new theoretical basis for revealing the regulatory mechanism of cell walls of plant pathogenic fungi and optimizing plant anti-fungal genetic engineering. The results showed that 211 genes (97 up-regulated genes and 114 down-regulated genes) were differentially expressed in sod1Δyeast cells compared with the wild type yeast cells after CFW treatment ( 10 μg/mL for 1 h ) . The microarray data were further confirmed by qPCR analysis,using five randomly selected,differentially expressed genes. The differentially expressed genes were mainly related to cell wall,cell metabolism,protein synthesis,cell de-fence,and included genes with unknown functions. Taken together,SOD1 deletion significantly affects the genome-wide transcriptional profile in response to the cell wall-perturing agent CFW in yeast.
Keywords：fungal cell wall-perturbing agentCalcofluor whiteSaccharomyces cerevisiaeDNA microar-raycell wallSOD
Publication Date：2016-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 76-79 )