Effects of Infection of Rose Plants by Rose Powdery Mildew on Activity of Acetylcholinesterase of Larvae of Beet Armyworm
YANG Fazhong
YANG Deqiang
YANG Bin
SUN Hao
Abstract：With spectrometry method,the changes of protein content and acetylcholinesterase( AChE) ac-tivity in the larvae of beet armyworm,Spodoptara exigua(Hübner),fed on rose leaves infected with rose powdery mildew,Sphaerotheca pannosa( Wallr.:Ex Fr. ) Lév. ,were determined in the present study,to provide reference for understanding the mechanism of plant-mediated indirect interaction between phyto-pathogenic fungi and insects. The beet armyworm was detrimentally influenced by infection of its host plants of rose,Rosa chinensis Jacq. Therefore,the protein content and the AChE activity of the larvae fed on mildew-infected rose leaves decreased. After the larvae were fed on infected rose leaves for nine days, the protein content decreased by 81. 77%(P<0. 01) and the AChE activity[8. 89 μmol/(mg·min)] decreased by 68. 03%(P<0. 01),when compared with larvae fed on healthy rose leaves. Also the Km ( Michaelis-Menten constant) and vmax ( maximum velocity of hydrolysis) of AChE were detrimentally af-fected[Km increased(P<0. 001) and vmax decreased(P<0. 001)],showing that the AChE activity de-creased when the larvae were fed with mildew-infected rose leaves. The obtained results from the aspect of insect physiology demonstrate that the inhibition of infection of rose plants by powdery mildew on beet ar-myworm is clearly related to the changes of protein content and activity of AChE in the larvae of beet ar-myworm.
Keywords：beet armywormRosa chinensisSphaerotheca pannosaacetylcholinesteraseinteraction be-tween fungus and insect
Publication Date：2015-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：4( 75-78 )