The Optimum Culture Condition of Actinomycete K13 and Its Inhibition on the Pathogen of Strawberry Gray Mold
XU Bin
CHEN Yinfeng
ZHANG Yun
WEI Lihui
ZHANG Qing
CHEN Xijun
Abstract：To realize the ecological control of strawberry gray mold,strain K13,one antagonistic actinomy-cete strain against the pathogen Botrytis cinerea,was isolated from the soil by confrontation culture,which could significantly inhibit the growth of many plant pathogenic fungi. The optimal condition of producing antifungal substance by K13 was inoculating the strain into Gause’ s medium with the initial pH value of 8. 0 at 28 ℃ with continuous shaking culture for 6 d. Results showed that K13 could inhibit the hypha growth of B. cinerea,with the inhibition rate of 55. 93% using colony diameter method. It could also inhi-bit the spore bearing,spore germination and germ-tube elongation of B. cinerea,and the inhibition rates were 72. 53%,65. 43% and 59. 83% respectively when the culture filtrate was diluted by two folds. Mi-croscopic observation indicated that the actinomycete filtrates could make the fungus hypha distortion,de-formation,fracture and even disintegration. All these mean that K13 can inhibit the growth and develop-ment of B. cinerea strongly and has the potential of being developed into bio-control agents.
Keywords：antagonistic actinomycete K13culture conditioninhibitioninhibitory spectrumBotrytis cinereainhibitory mechanism
Publication Date：2015-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 70-74 )