Fungal Cell Wall-perturbing Agent Congo Red-induced Yeast Apoptosis and Its Underlying Mechanisms
ZHANG Xiaohua
SUN Yeying
BIAN Weihua
XU Cong
WU Yuyong
LIU Xiangyong?
Abstract：This paper investigated the effect of fungal cell wall-perturbing agent Congo red ( CR ) treat-ment on apoptosis of Saccharomyces cerevisiae and its underlying mechanisms,to provide a new theoretical foundation for study of plant pathogenic fungi apoptosis in future. The results showed that the CR-treated yeast cells displayed more serious apoptosis compared with the normal control group,including decrease of cell survival rate,increase of reactive oxygen species,phosphatidylserine externalization,and nuclei lysis. The CR-induced yeast apoptosis was suppressed by addition of antioxidant N-acetyl-L-cysteine ( NAC ) , overexpression of Cu,Zn-superoxide dismutase encoding gene SOD1 or deletion of YCA1 gene. Taken to-gether,CR could induce ROS and Yca1p-dependent yeast apoptosis.
Keywords：cell wallCongo redSaccharomyces cerevisiaeapoptosiscell wall-perturbing agents
Publication Date：2015-01-01
Online Publishing Date：2026-09-12(First online date of this platform, not the publication date of the document)
Pages：5( 65-69 )