Screening and Analysis of Potato StRab Interacting Proteins
GAO Wen
XIE Conghua
Abstract:To further explore the functional mechanism of StRab protein in potato late blight resistance, StRab was constructed into the pGBKT7 vector as bait protein, and then used to screen the interacting proteins by yeast two-hybrid technology in potato( Solanum tuberosum L. ) cDNA library. Twenty-six can-didate clones were acquired by evaluation,sequencing and Blast analysis. Of the potential target proteins, scaffold protein,polyubiquitin,ERF transcription factor,proteinase inhibitor,polyphenol oxidase and chiti-nase were reported to relate with adversity stresses in plant. The results suggested that the StRab protein might play an important role in signal pathway of potato late blight resistance.
Keywords:potatoStRabyeast two-hybridinteracting proteins
Publication Date:2015-12-01
Online Publishing Date:2026-09-12(First online date of this platform, not the publication date of the document)
Pages:5( 6-10 )
Journal of Henan Agricultural Sciences

Journal of Henan Agricultural Sciences

ISTICPKUCSCD
ISSN:1004-3268
Year, Vol.(Issue):2015,(12)