Preliminary Studies on Antifungal Activity of Two Chinese Herbal Medicinal Plant Extracts

ZHONG Hui; ZHONG Yong; QING Zhen; ZHOU Qiuyan; DENG Yecheng

doi:10.15933/j.cnki.1004-3268.2015.09.016

Abstract：A growth rate method was used for determining the antifungal activity of methanol extracts from two species of Chinese herbal medicine against ten species of plant pathogenic fungi.The results showed that the methanol extract from Atractylodes lancea ( Thunb.) DC.had a high inhibitory activity against Exserohilum turcicum ( Pass ) Leonard et Suggs, Diaporthe citri ( Fawcett ) Wolf, Ceratocystis paradoxa (Dode)Moreau,Pestalotiopsis theae(Sawada)Steyaert,Phytophthora parasitica var.Nicotianae(Breda de Hean)Tucker,with the inhibition rates of 85.17%—100%at 10 g/L.EC50 values of the methanol extract from Atractylodes lancea(Thunb.)DC.against Exserohilum turcicum(Pass)Leonard et Suggs,Ceratocystis paradoxa(Dode)Moreau,Pestalotiopsis theae(Sawada)Steyaert,Cochliobolus miyabeanus(Ito et Kubibay) Drechsler et Dastur,Colletotrichum capsici( syd.) Butl,Phytophthora parasitica var.Nicotianae( Breda de Hean) Tucker were 0.131 6—0.980 2 g/L.The methanol extract from Lindera aggregata ( Sims ) Kosterm.had a high inhibitory activity against ten species of plant pathogenic fungi, with the inhibition rates of more than 80% at 10 g/L, and with the EC50 values of 0.102 0—0.632 9 g/L against nine species among them except Alternaria citri.The active ingredients of Atractylodes lancea( Thunb.) DC.and Lindera aggregata( Sims) Kosterm.were preliminarily partitioned respectively by liquid-liquid and solid-liquid extraction and activity-directed isolation methods, and the results indicated that the active ingredients of the former mainly consisted in the ethyl acetate and petroleum ether fraction,and the latter’s in the ethyl acetate fraction.